| # | Title | Artist | Length |
|---|
| 1 | Mississippi Boweavil Blues | Charley Patton | 3:06 |
| 2 | My Black Mama, Part 1 | Son House | 3:12 |
| 3 | Future Blues | Willie Brown | 3:02 |
| 4 | Canned Heat | Tommy Johnson | 3:28 |
| 5 | Left Alone Blues | Ishmon Bracey | 3:23 |
| 6 | Preachin' Blues (Up Jumped the Devil) | Robert Johnson | 2:53 |
| 7 | Kind Hearted Woman Blues | Robert Johnson | 2:53 |
| 8 | Wind Howlin' Blues | Honey Boy Edwards | 3:16 |
| 9 | Devil Got My Woman | Skip James | 3:01 |
| 10 | Shake 'em on Down | Bukka White | 3:03 |
| 11 | Sitting on Top of the World | Mississippi Sheiks | 3:06 |
| 12 | Stack O'Lee Blues | Mississippi John Hurt | 2:59 |
| 13 | Barrelhouse Blues | Edward Andrews | 2:54 |
| 14 | Catfish Blues | Robert Petway | 2:51 |
| 15 | Bottle It Up and Go | Tommy McClennan | 2:50 |
| 16 | Bull Frog Blues | William Harris | 3:09 |
| 17 | Mule Skinner Blues | Jimmie Rodgers | 3:01 |
| 18 | Farewell Blues | Sol Hoopi | 2:51 |
| 19 | Old Original Kokomo Blues | Kokomo Arnold | 2:55 |
| 20 | Country Blues | Muddy Waters | 3:28 |
| 21 | I Be's Troubled | Muddy Waters | 3:07 |
| 22 | Boogie Chillin | John Lee Hooker | 3:13 |
| 23 | Little Boy Blue | Robert Lockwood, Jr. | 3:03 |
| 24 | Ramblin' | Johnny Shines | 2:34 |
| 25 | Yellow Dog Blues | Sam Collins | 2:31 |