| # | Title | Artist | Length |
|---|
| 1 | O Death | Pace Jubilee Singers | 3:28 |
| 2 | Hello Stranger | The Carter Family | 2:47 |
| 3 | Sitting on Top of the World | Mississippi Sheiks | 3:16 |
| 4 | I'm So Glad | Skip James | 2:52 |
| 5 | Gathering Flowers for the Master's Bouquet | The Stanley Brothers | 2:54 |
| 6 | I'm a Man of Constant Sorrow | Emry Arthur | 3:21 |
| 7 | Sail Away Ladies | Uncle Dave Macon | 2:57 |
| 8 | You've Got to Move | The Fairfield Four | 2:30 |
| 9 | Heaven's Radio | Molly O’Day & The Cumberland Mountain Folks | 2:52 |
| 10 | Dark Was the Night (Cold Was the Ground) | Blind Willie Johnson | 3:22 |
| 11 | Waiting for a Train | Jimmie Rodgers | 2:46 |
| 12 | Brown's Ferry Blues | The Delmore Brothers | 2:37 |
| 13 | Old Country Stomp | Henry Thomas | 2:55 |
| 14 | Honey in the Rock | The Five Blind Boys of Alabama | 2:33 |
| 15 | Don't Let Your Deal Go Down Blues | Charlie Poole | 2:53 |
| 16 | Soldier's Joy | Gid Tanner & His Skillet Lickers | 2:43 |
| 17 | Parchman Farm Blues | Bukka White | 2:40 |
| 18 | Corrina, Corrina | Clarence Ashley | 3:05 |
| 19 | I Wish I Was a Mole in the Ground | Bascom Lamar Lunsford | 3:22 |
| 20 | Avalon Blues | Mississippi John Hurt | 3:05 |
| 21 | Old Rub Alcohol Blues | Dock Boggs | 3:08 |
| 22 | O Death | Charlie Monroe's Boys | 2:05 |
| 23 | No Depression in Heaven | The Carter Family | 2:56 |
| 24 | I'm a Man of Constant Sorrow | The Stanley Brothers | 2:56 |