| # | Title | Artist | Length |
|---|
| 1 | I Am a Man of Constant Sorrow | The Stanley Brothers | 2:58 |
| 2 | John Henry | Bailey Brothers | 2:42 |
| 3 | Earl’s Breakdown | Flatt & Scruggs | 3:00 |
| 4 | In the Pines | Bill Monroe | 3:23 |
| 5 | There’s Another Baby Waiting for Me Down the Line | Reno and Smiley | 2:29 |
| 6 | Hold the Woodpile Down | Uncle Dave Macon | 3:03 |
| 7 | Bluegrass Breakdown | Bill Monroe | 2:19 |
| 8 | Going Back to the Blue Ridge Mountains | Delmore Brothers | 2:49 |
| 9 | Hawkins Rag | Gid Tanner & His Skillet Lickers | 2:49 |
| 10 | Molly & Tenbrooks | The Stanley Brothers | 2:25 |
| 11 | Dim Light Thick Smoke | Flatt & Scruggs | 2:54 |
| 12 | Mule Skinner Blues | Bill Monroe | 2:44 |
| 13 | Are You From Dixie | The Blue Sky Boys | 2:43 |
| 14 | Tennessee Cut -Up Breakdown | Reno and Smiley | 1:42 |
| 15 | The Little Glass of Wine | The Stanley Brothers | 2:55 |
| 16 | Beautiful Brown Eyes | Bailey Brothers | 2:45 |
| 17 | Blue Yodel No. 4 | Bill Monroe | 2:43 |
| 18 | Footprints in the Snow | Cliff Carlisle | 1:59 |
| 19 | Foggy Mountain Special | Flatt & Scruggs | 3:39 |
| 20 | Sally Goodin | Ernest V. Stoneman and His Dixie Mountaineers | 3:04 |