| # | Title | Artist | Length |
|---|
| 1 | Catfish Blues | Robert Petway | 2:55 |
| 2 | Baby, Please Don't Go | Big Joe Williams | 3:25 |
| 3 | Ham an' Eggs | Lead Belly | 3:02 |
| 4 | Mississippi River Blues | Big Bill Broonzy | 2:43 |
| 5 | Just a Good Woman Through With the Blues | Trixie Butler | 2:44 |
| 6 | Garbage Man Blues | Milton Brown and His Musical Brownies | 2:46 |
| 7 | The Panama Limited | Bukka White | 3:15 |
| 8 | Cool Drink of Water Blues | Tommy Johnson | 3:36 |
| 9 | The Midnight Special | Lead Belly | 3:10 |
| 10 | Worried Man Blues | The Carter Family | 2:47 |
| 11 | Les Blues de Voyage | Amédé Ardoin & Dennis McGee | 2:57 |
| 12 | K. C. Railroad Blues | Andrew & Jim Baxter | 3:32 |
| 13 | Somebody's Been Stealin' | Rev. J.M. Gates | 2:50 |
| 14 | Beale Street Blues | Alberta Hunter | 3:18 |
| 15 | Devil in the Wood Pile | Noah Lewis | 2:58 |
| 16 | Walk Right In | Cannon’s Jug Stompers | 2:59 |
| 17 | Ninety-nine Year Blues | Julius Daniels | 3:06 |
| 18 | Got Cut All to Pieces | Bessie Tucker | 3:30 |
| 19 | Feather Bed | Cannon’s Jug Stompers | 3:20 |
| 20 | Can't Put a Bridle on that Mule this Morning | Julius Daniels | 3:27 |
| 21 | Davidson County Blues | DeFord Bailey | 3:24 |
| 22 | Frankie and Johnny | Frank Crumit | 3:22 |
| 23 | Dixie Bo-Bo | Taskiana Four | 3:05 |
| 24 | Sometimes I feel like a motherless child | Paul Robeson | 3:04 |
| 25 | St. Louis Blues | Hall Johnson Choir | 3:56 |