| # | Title | Artist | Length |
|---|
| 1 | An Excerpt From "Railroad Dynamics" | [no artist] | 0:24 |
| 2 | Train 45 | The New Lost City Ramblers | 2:18 |
| 3 | Kassie Jones | Furry Lewis | 2:56 |
| 4 | Jay Gould's Daughter | Pete Seeger | 2:38 |
| 5 | Railroad Bill | Walt Robertson | 2:08 |
| 6 | Linin' Track | Lead Belly | 1:15 |
| 7 | Freight Train | Elizabeth Cotten | 2:43 |
| 8 | Drill, Ye Tarriers, Drill | Cisco Houston | 2:30 |
| 9 | Zack, The Mormon Engineer | L. M. Hilton | 2:02 |
| 10 | Lost Train Blues | The Virginia Mountain Boys | 2:57 |
| 11 | The F.F.V. | Annie Watson | 3:52 |
| 12 | He's Coming To Us Dead | The New Lost City Ramblers | 3:15 |
| 13 | The Train That Carried My Girl From Town | Doc Watson | 2:18 |
| 14 | Rock Island Line | Lead Belly | 2:03 |
| 15 | Lonesome Train | Sonny Terry, Woody Guthrie And Cisco Houston | 3:31 |
| 16 | John Henry | Woody Guthrie And Cisco Houston | 2:42 |
| 17 | The Wreck Of Number Nine | Rosalie Sorrels | 1:36 |
| 18 | Freight Train Blues | Brownie McGhee | 3:36 |
| 19 | The New Market Wreck | Mike Seeger | 3:39 |
| 20 | Jerry, Go Oil That Car | Harry “Haywire Mac” McClintock | 2:38 |
| 21 | Way Out In Idaho | Rosalie Sorrels | 3:33 |
| 22 | Old Henry Died On The Mountain | Henry Grady Terrell | 1:55 |
| 23 | Casey Jones | John D. Mounce | 0:20 |
| 24 | Wreck Of The Old 97 | Pop Stoneman | 2:51 |
| 25 | Midnight Special | Lead Belly | 2:03 |
| 26 | Wabash Cannonball | Doc Watson | 3:17 |
| 27 | Lost Train Blues | Vernon Sutphin | 1:13 |
| 28 | New River Train | Iron Mountain String Band | 4:26 |
| 29 | Excerpt From "Three Little Engines And 33 Cars" | [no artist] | 0:25 |
