| # | Title | Artist | Length |
|---|
| 1 | Killing Floor | Howlin’ Wolf | 2:50 |
| 2 | Don't Go No Farther | Muddy Waters | 2:53 |
| 3 | I'm a King Bee | Slim Harpo | 3:01 |
| 4 | Need Your Love So Bad | Little Willie John | 2:14 |
| 5 | Sittin' on Top of the World | Mississippi Sheiks | 3:12 |
| 6 | Corrinne Corrinna | Bo Carter | 3:16 |
| 7 | Shine on Me | Ernest Phipps & His Holiness Singers | 3:02 |
| 8 | Judgement | Sister Mary Nelson | 2:23 |
| 9 | John the Revelator | Blind Willie Johnson | 3:17 |
| 10 | Evil (Is Going on) | Howlin’ Wolf | 2:53 |
| 11 | Baby Please Don't Go | Lightnin’ Hopkins | 2:51 |
| 12 | Nobody's Fault but Mine | Blind Willie Johnson | 3:09 |
| 13 | Dust My Broom | Elmore James | 2:44 |
| 14 | Key to the Highway | Big Bill Broonzy | 3:01 |
| 15 | No Depression (In Heaven) | The Carter Family | 2:52 |
| 16 | When the Levee Breaks | Memphis Minnie | 3:08 |
| 17 | Shake 'em on Down | Bukka White | 3:01 |
| 18 | Jesus Make Up My Dying Bed | Blind Willie Johnson | 3:11 |
| 19 | Smokestack Lightning | Howlin’ Wolf | 3:05 |
| 20 | (I'm Your) Hootchie Coochie Man- recorded in:
- Chicago, Illinois, United States (on 1954-01-07)
- double bass:
- Willie Dixon (on 1954-01-07)
- drums (drum set):
- Fred Below (on 1954-01-07)
- guitar:
- Muddy Waters (on 1954-01-07) and Jimmy Rogers (on 1954-01-07)
- harmonica:
- Little Walter (on 1954-01-07)
- piano:
- Otis Spann (on 1954-01-07)
- vocals:
- Muddy Waters (on 1954-01-07)
- phonographic copyright (℗) by:
- MCA Records, Inc. (in 1954)
- part of:
- Blues Hall of Fame: Classic of Blues Recording Single (number: 1984), Rock and Roll Hall of Fame – 500 Songs That Shaped Rock and Rolling Stone: 500 Greatest Songs of All Time (number: 225)
- recording of:
- I’m Your Hoochie Coochie Man (on 1954-01-07)
- lyricist and composer:
- Willie Dixon
- publisher:
- Arc Music, Arc Music Corp., Bug Music Ltd., Campbell Connelly, Hello Mr. Wilson, Hoochie Coochie Music, Jewel Music Pub Co Ltd and Willie Dixon Music
| Muddy Waters | 2:47 |