| # | Title | Artist | Length |
|---|
| 1 | Po’ Lazarus | James Carter & The Prisoners | 4:31 |
| 2 | Big Rock Candy Mountain | Harry McClintock | 2:18 |
| 3 | You Are My Sunshine | Norman Blake | 4:28 |
| 4 | Down to the River to Pray- recording engineer and mixer:
- Mike Piersante
- assistant engineer:
- Matt Andrews and Rail Rogut
- producer:
- T‐Bone Burnett
- assistant mixer:
- Ok Hee Kim
- additional vocals:
- Norman Blake, Dub Cornett, Pat Enright, Porter McClister, Tim O’Brien, Maura O’Connell, Sam Phillips, David Rawlings and Gillian Welch
- choir vocals:
- The First Baptist Choir of White House, Tennessee
- lead vocals:
- Alison Krauss
- phonographic copyright (℗) by:
- Mercury Records (in 2000)
- recorded at:
- Sound Emporium Studios in Nashville, Tennessee, United States
- recording of:
- The Good Old Way
- writer:
- [traditional]
| Alison Krauss | 2:56 |
| 5 | I Am a Man of Constant Sorrow (radio station version) | The Soggy Bottom Boys feat. Dan Tyminski | 3:10 |
| 6 | Hard Time Killing Floor Blues | Chris Thomas King | 2:42 |
| 7 | I Am a Man of Constant Sorrow (instrumental) | Norman Blake | 4:29 |
| 8 | Keep on the Sunny Side | The Whites | 3:34 |
| 9 | I’ll Fly Away | Alison Krauss and Gillian Welch | 3:58 |
| 10 | Didn’t Leave Nobody but the Baby | Emmylou Harris, Alison Krauss and Gillian Welch | 1:58 |
| 11 | In the Highways | Sarah, Hannah and Leah Peasall | 1:36 |
| 12 | I Am Weary (Let Me Rest) | The Cox Family | 3:14 |
| 13 | I Am a Man of Constant Sorrow (instrumental) | John Hartford | 2:34 |
| 14 | O Death | Ralph Stanley | 3:20 |
| 15 | In the Jailhouse Now | The Soggy Bottom Boys feat. Tim Blake Nelson | 3:36 |
| 16 | I Am a Man of Constant Sorrow (with band) | The Soggy Bottom Boys feat. Dan Tyminski | 4:17 |
| 17 | Indian War Whoop | John Hartford | 1:30 |
| 18 | Lonesome Valley | The Fairfield Four | 4:07 |
| 19 | Angel Band | The Stanley Brothers | 2:18 |