| # | Title | Artist | Length |
|---|
| 1 | When the Levee Breaks | Kansas Joe & Memphis Minnie | 3:12 |
| 2 | Kokomo Blues | Scrapper Blackwell | 3:03 |
| 3 | Stack O’Lee Blues | Ma Rainey | 2:54 |
| 4 | West Coast Blues | Blind Blake | 3:14 |
| 5 | Fishing Blues | Henry Thomas | 2:45 |
| 6 | Stealin’, Stealin’ | Memphis Jug Band | 2:59 |
| 7 | T.B. Blues | Victoria Spivey | 3:14 |
| 8 | See That My Grave Is Kept Clean | Blind Lemon Jefferson | 2:54 |
| 9 | Cow Cow Blues | Cow Cow Davenport | 3:11 |
| 10 | Mother’s Children Have a Hard Time | Blind Willie Johnson | 3:22 |
| 11 | Brown Skin Blues | Dick Justice | 3:14 |
| 12 | Devil and My Brown Blues | Bo Weavil Jackson | 2:51 |
| 13 | Got the Blues, Can’t Be Satisfied | Mississippi John Hurt | 2:52 |
| 14 | Roll and Tumble Blues | Hambone Willie Newbern | 3:02 |
| 15 | Pinetop’s Boogie Woogie | Clarence “Pine Top” Smith | 3:14 |
| 16 | Section Gang Blues | Texas Alexander | 2:56 |
| 17 | Statesboro Blues | Blind Willie McTell | 2:33 |
| 18 | The Dirty Dozen | Speckled Red | 3:14 |
| 19 | Screamin’ and Hollerin’ the Blues | Charley Patton | 3:09 |
| 20 | Blue Yodel no. 1 (T for Texas) | Jimmie Rodgers | 3:26 |
| 21 | St. Louis Blues | Bessie Smith | 3:11 |
| 22 | All I Want Is a Spoonful | Papa Charlie Jackson | 2:40 |
| 23 | Bottleneck Blues | Weaver & Beasley | 2:54 |
| 24 | It’s Tight Like That no. 2 | Tampa Red & Georgia Tom | 2:43 |
| 25 | Canned Heat Blues | Tommy Johnson | 3:37 |