| # | Title | Artist | Length |
|---|
| 1 | Mississippi River Blues | Big Bill Broonzy | 2:46 |
| 2 | Ain’t No Telling | Mississippi John Hurt | 2:58 |
| 3 | Special Steam Lines | Bukka White | 2:56 |
| 4 | I’m Getting Wild About Her | Big Joe Williams | 2:49 |
| 5 | Hellhound on My Trail | Robert Johnson | 2:38 |
| 6 | Cool Drink of Water Blues | Tommy Johnson | 3:36 |
| 7 | Take a Little Walk With Me | Robert Lockwood | 2:55 |
| 8 | I Be’s Troubled | Muddy Waters | 3:06 |
| 9 | Shady Grove Blues | John Lee Hooker | 3:22 |
| 10 | Hobo Jungle Blues | Sleepy John Estes | 2:57 |
| 11 | 34 Blues | Charley Patton | 2:58 |
| 12 | Left Alone Blues | Ishman Bracey | 3:30 |
| 13 | Highway No.51 | Tommy McClennan | 2:51 |
| 14 | Rockin’ Chair Blues | Robert Petway | 2:56 |
| 15 | My Black Mama, Part 1 | Son House | 3:10 |
| 16 | Nobody’s Fault but Mine | Blind Willie Johnson | 3:11 |
| 17 | Dirty Mistreater | Willie “Poor Boy” Lofton | 2:50 |
| 18 | Stagolee | David “Honeyboy” Edwards | 1:07 |
| 19 | Moon Going Down | Charley Patton | 3:17 |
| 20 | Devil Got My Woman | Skip James | 3:01 |
| 21 | Preaching Blues (Up Jumped the Devil) | Robert Johnson | 2:53 |
| 22 | Delta Blues | Big Joe Williams | 2:41 |
| 23 | Black Pony Blues | Arthur “Big Boy” Crudup | 3:23 |
| 24 | Frankie | Mississippi John Hurt | 3:25 |
| 25 | My Pencil Won’t Write No More | Bo Carter | 2:56 |