| # | Title | Artist | Length |
|---|
| 1 | Hard Time Blues | Lane Hardin | 3:21 |
| 2 | Down the Dirt Road Blues | Charley Patton | 2:57 |
| 3 | Poor Boy, Long Ways From Home | Banjo Joe | 3:12 |
| 4 | Hastings Street | Blind Blake & Charlie Spand | 3:15 |
| 5 | See That My Grave’s Kept Clean | Blind Lemon Jefferson | 2:55 |
| 6 | Gonna Have ’Lasses in the Morning | Golden Melody Boys | 2:43 |
| 7 | New Orleans Stop Time | Memphis Minnie & Bumble Bee Slim | 2:59 |
| 8 | Prove It On Me Blues | Ma Rainey | 2:43 |
| 9 | Gonne Die With My Hammer in My Hand | The Williamson Brothers & Curry | 3:26 |
| 10 | Last Kind Words Blues | Geeshie Wiley | 3:04 |
| 11 | Banjoreno | Dixieland Jug Blowers | 3:15 |
| 12 | It’s a Good Thing | Beale Street Sheiks | 3:01 |
| 13 | Trust in God and Do the Right | Blind Willie Davis | 2:50 |
| 14 | Someday Baby Blues | Sleepy John Estes | 3:03 |
| 15 | Lonesome Road Blues | Sam Collins | 3:07 |
| 16 | Future Blues | Willie Brown | 2:58 |
| 17 | Bull Doze Blues | Henry Thomas | 3:29 |
| 18 | Brown Skin Gal (Down the Lane) | Massey Family | 2:47 |
| 19 | Henry Lee a.k.a. Young Hunting | Dick Justice | 3:26 |
| 20 | Old Country Rock | William Moore | 3:02 |
| 21 | La Danseuse | Delma Lachney & Blind Uncle Gaspard | 2:56 |
| 22 | My Black Mama, Parts 1 & 2 | Son House | 6:24 |
| 23 | Cypress Grove Blues | Skip James | 3:16 |