| # | Title | Artist | Length |
|---|
| 1 | Foggy Mountain Breakdown | Lester Flatt & Earl Scruggs | 2:40 |
| 2 | I'm Man of Constant Sorrow | The Stanley Brothers | 2:58 |
| 3 | Blue Moon of Kentucky | Bill Monroe and the Bluegrass Boys | 2:00 |
| 4 | Garden in the Sky | The Blue Sky Boys | 2:45 |
| 5 | Tennessee Cut Up Breakdown | Reno and Smiley | 1:40 |
| 6 | Lonesome, Sad and Blue | The Lonesome Pine Fiddlers | 2:24 |
| 7 | Will the Circle Be Unbroken? | Brown’s Ferry Four | 2:56 |
| 8 | Are You Missing Me? | Jim and Jesse | 2:24 |
| 9 | Cripple Creek | Sonny Terry & Brownie McGhee | 4:45 |
| 10 | You Can't Do Wrong and Get By | The Delmore Brothers | 2:42 |
| 11 | Sunny Side of the Mountain | Wilma Lee & Stoney Cooper | 3:04 |
| 12 | Higher in My Prayer | Molly O’Day & The Cumberland Mountain Folks | 2:27 |
| 13 | Tomorrow May Be Different | Jim Eanes & The Shenandoah Valley Boys | 2:38 |
| 14 | Going Back to Old Kentucky | Hobo Jack Adkins | 2:05 |
| 15 | Molly and Tenbrooks | Bill Monroe and the Bluegrass Boys | 2:45 |
| 16 | Crazy Finger Blues | Reno and Smiley | 2:43 |
| 17 | Goin' to the Barn Dance Tonight | Carson Robison and His Pioneers | 2:45 |
| 18 | Ida Red | The Blue Sky Boys | 1:20 |
| 19 | Old Salty Dog Blues | Lester Flatt & Earl Scruggs | 2:26 |
| 20 | Keep on the Sunny Side | The Carter Family | 2:49 |
| 21 | Little Birdie | Wade Mainer, Zeke Morris & Steve Ledford | 2:47 |
| 22 | I Cried Again | Jim Eanes & The Shenandoah Valley Boys | 3:06 |
| 23 | Come Walk With Me | Wilma Lee & Stoney Cooper | 2:24 |
| 24 | Beautiful Brown Eyes | Bailey Brothers | 2:43 |
| 25 | The Fields Have Turned Brown | The Stanley Brothers | 2:33 |