| # | Title | Artist | Length |
|---|
| 1 | I Know His Blood Can Make Me Whole | Blind Willie Johnson | 3:06 |
| 2 | Bath House Blues | Ashley's Melody Men | 2:53 |
| 3 | Worried Blues | Frank Hutchison | 3:19 |
| 4 | Snake Doctor Blues | J.D. Short | 3:26 |
| 5 | Acorn Stomp | East Texas Serenaders | 2:53 |
| 6 | Sal Got a Meatskin | Carlisle Brothers | 2:54 |
| 7 | Streetcar Blues | Sleepy John Estes | 3:16 |
| 8 | Fort Smith Breakdown | Luke Highnight & His Ozark Strutters | 2:47 |
| 9 | Sleepy Desert | Wilmer Watts & The Lonely Eagles | 3:06 |
| 10 | Walking Blues | Son House | 2:57 |
| 11 | Sweet Rivers | J.T. Allison’s Sacred Harp Singers | 3:12 |
| 12 | Gonna Die With My Hammer in My Hand | Williamson Brothers and Curry | 3:26 |
| 13 | Mean Black Cat | Charley Patton | 2:57 |
| 14 | Billy in the Lowground | Lowe Stokes | 3:05 |
| 15 | Good Time Blues | Jelly Roll Anderson | 2:44 |
| 16 | Christmas Time Will Soon Be Over | Fiddlin’ John Carson and His Virginia Reelers | 2:53 |
| 17 | Steel Driving Man | Fruit Jar Guzzlers | 3:05 |
| 18 | I'm So Glad | Skip James | 2:50 |
| 19 | Rock About My Sara Jane | Uncle Dave Macon & His Fruit Jar Drinkers | 3:24 |
| 20 | I'm Taking My Audition to Sing Up in the Sky | Cap, Andy & Flip | 3:11 |
| 21 | Undertaker Blues | Cloe's Washboard Band | 3:04 |
| 22 | Sally Johnson | Ocar Harper's Texas String Band | 2:34 |
| 23 | I'll Stay on the Right Road Now | Fa Sol La Singers | 2:49 |