| # | Title | Artist | Length |
|---|
| 1 | I’m a Man of Constant Sorrow | Emry Arthur | 3:23 |
| 2 | Don’t Let Your Deal Go Down Blues | Charlie Poole & The North Carolina Ramblers | 2:55 |
| 3 | Going Back to Jericho | Dock Walsh | 2:24 |
| 4 | Backwater Blues | Uncle Dave Macon & Sam McGee | 3:16 |
| 5 | Hard for to Love | Hayes Shepherd | 3:14 |
| 6 | Sail Away Ladies | Uncle Dave Macon & His Fruit Jar Drinkers | 2:59 |
| 7 | Carolina Stompdown | Aiken County String Band | 3:00 |
| 8 | Train 45 | Grayson & Whitter | 3:06 |
| 9 | Go Along Mule | Uncle Dave Macon & His Fruit Jar Drinkers | 3:06 |
| 10 | Little Maggie With a Dram Glass in Her Hand | Grayson & Whitter | 3:30 |
| 11 | All Night Long | Earl Johnson & His Clodhoppers | 3:16 |
| 12 | Three Men Went A’Hunting | Byrd Moore and His Hot Shots | 2:37 |
| 13 | I’ll Roll in My Sweet Baby’s Arms | Buster Carter & Preston Young | 3:00 |
| 14 | Seven and a Half | J.E. Mainer’s Mountaineers | 3:09 |
| 15 | New River Train | The Monroe Brothers | 3:42 |
| 16 | Short Life and It’s Trouble | Wade Mainer & Zeke Morris | 2:28 |
| 17 | I’m Bound to Ride | Arthur Smith and the Delmore Brothers | 2:23 |
| 18 | Two Italians… Red Bird | Monroe Gevedon and Family | 2:15 |
| 19 | I Won’t Be Worried | Wade Mainer and The Sons of the Mountaineers | 2:36 |
| 20 | Big at the Little, Bottom at the Top | Bill Carlisle’s Kentucky Boys | 3:11 |
| 21 | Let Me Be Your Salty Dog | The Morris Brothers | 2:40 |
| 22 | Footprints in the Snow | Cliff Carlisle's Buckle Busters | 2:46 |
| 23 | Blue Ridge Mountain Blues | J.E. Mainer’s Mountaineers | 2:33 |
| 24 | When I Lay My Burden Down | Roy Acuff and His Smoky Mountain Boys | 2:58 |
| 25 | Orange Blossom Special | Rouse Brothers | 2:58 |
