| # | Title | Artist | Length |
|---|
| 1 | On the Road Again | Memphis Jug Band | 2:52 |
| 2 | Dark Night Blues | Blind Willie McTell | 2:55 |
| 3 | Minglewood Blues | Cannon’s Jug Stompers | 3:42 |
| 4 | Hard Time Killin' Floor Blues | Skip James | 2:50 |
| 5 | I'm Gonna Cross the River of Jordan Some O' These Days | Jaybird Coleman | 3:08 |
| 6 | High Water Everywhere | Charley Patton | 3:08 |
| 7 | I Got Mine | Frank Stokes | 3:07 |
| 8 | Sugar Baby | Dock Boggs | 3:00 |
| 9 | Big Bend Gal | Shelor Family | 2:49 |
| 10 | The Peddler and His Wife | Hayes Shepherd | 3:22 |
| 11 | Little Rabbit | Crockett's Kentucky Mountaineers | 3:01 |
| 12 | All Night Long Blues | Burnett & Rutherford | 3:15 |
| 13 | Mineola Rag | East Texas Serenaders | 2:44 |
| 14 | Greenback Dollar | Weems String Band | 3:09 |
| 15 | Kater Street Rag | Bennie Moten’s Kansas City Orchestra | 2:34 |
| 16 | Sobbin' Blues | King Oliver’s Creole Jazz Band with Louis Armstrong, Johnny Dodds & Lil Hardin | 3:19 |
| 17 | Mojo Strut | Pickett-Parham Apollo Syncopators with "Tiny" Parham & Junie C. Cobb | 2:49 |
| 18 | Somebody Stole My Gal | Frankie Franko & His Louisianians with Ernest "Punch" Miller | 2:30 |
| 19 | Wild Cat Blues | Clarence Williams' Blue Five with Sidney Bechet | 3:02 |
| 20 | Kansas City Stomps | Jelly Roll Morton’s Red Hot Peppers | 2:56 |
| 21 | King Joe | Jimmie Noone | 3:01 |