| # | Title | Artist | Length |
|---|
| 1 | Orange Blossom Special | Bill Monroe | 2:35 |
| 2 | Muleskinner Blues | Bill Monroe | 2:45 |
| 3 | Uncle Penn | Bill Monroe | 2:46 |
| 4 | The Great Speckled Bird | Charlie Monroe | 2:14 |
| 5 | Don't Get Above Your Raisin' | Lester Flatt & Earl Scruggs | 2:43 |
| 6 | Earl's Breakdown | Lester Flatt & Earl Scruggs | 3:02 |
| 7 | The White Dove | The Stanley Brothers | 3:15 |
| 8 | Pretty Polly | The Stanley Brothers | 2:52 |
| 9 | I Am a Man of Constant Sorrow | The Stanley Brothers | 2:58 |
| 10 | Hawkins Rag | Gid Tanner & His Skillet Lickers | 2:50 |
| 11 | Sally Goodin | Ernest Stoneman | 3:41 |
| 12 | I'm the Talk of the Town | Reno and Smiley | 2:44 |
| 13 | Tennesse Cut Up Breakdown | Reno and Smiley | 1:42 |
| 14 | Feuding Banjos | Don Reno & Arthur Smith | 1:59 |
| 15 | Blues Stay Away from Me | The Delmore Brothers | 2:50 |
| 16 | Chalk Up Another One | Jimmy Martin & The Osborne Brothers | 2:37 |
| 17 | 20/20 Vision | Jimmy Martin & The Osborne Brothers | 2:43 |
| 18 | Doin' My Time | Lester Flatt & Earl Scruggs | 2:50 |
| 19 | Foggy Mountain Breakdown | Lester Flatt & Earl Scruggs | 2:42 |
| 20 | Blue Moon of Kentucky | Bill Monroe | 3:02 |