| # | Title | Artist | Length |
|---|
| 1 | You Are My Sunshine | The Rice Brothers Gang | 2:45 |
| 2 | Big Rock Candy Mountain | Burl Ives | 2:39 |
| 3 | Keep On The Sunny Side | The Carter Family | 2:51 |
| 4 | I Am A Man Of Constant Sorrow | Emry Arthur | 3:21 |
| 5 | Sail Away Ladies | Uncle Dave Macon | 2:57 |
| 6 | When The Saints Go Marching In | Fiddlin’ John Carson & Moonshine Kate | 3:06 |
| 7 | Red Wing | Riley Puckett | 2:41 |
| 8 | The Runaway Train | Vernon Dalhart | 3:14 |
| 9 | Wildwood Flower | The Carter Family | 3:11 |
| 10 | Ida Red | The Blue Sky Boys with Curley Parker | 1:21 |
| 11 | Little Bessie | The Alabama Barnstormers | 3:18 |
| 12 | I'm Thinking Tonight Of My Blue Eyes | The Carter Family | 3:04 |
| 13 | Backwater Blues | Uncle Dave Macon | 3:14 |
| 14 | Rock Island Line | Lead Belly | 2:35 |
| 15 | John Henry | Woody Guthrie & Cisco Houston | 2:45 |
| 16 | Mule Skinner's Blues | Bill Monroe and the Bluegrass Boys | 2:45 |
| 17 | Brown's Ferry Blues | The Delmore Brothers | 2:36 |
| 18 | Why Should It End This Way? | The Blue Sky Boys | 2:39 |
| 19 | Wabash Cannonball | Roy Acuff | 2:49 |
| 20 | Abbeville Breakdown | Alley Boys of Abbeville | 2:26 |
| 21 | My Little Lady | Jimmie Rodgers | 3:05 |
| 22 | I'm Goin' Away In The Morn | Uncle Dave Macon | 3:07 |
| 23 | Hard For To Love | Hayes Shepherd | 3:12 |
| 24 | Two Italians... Red Bird | Monroe Gevedon and Family | 2:14 |
| 25 | Great Speckled Bird | Roy Acuff | 2:54 |