| # | Title | Artist | Length |
|---|
| 1 | Keep on the Sunny Side | Doc Watson | 2:08 |
| 2 | I Don't Believe You've Met My Baby | The Louvin Brothers | 2:07 |
| 3 | Cotton Fields (The Cotton Song) | Mac Wiseman | 2:00 |
| 4 | Corina, Corina | The Blue Sky Boys | 2:25 |
| 5 | Wabash Cannonball | Roy Acuff and His Smoky Mountain Boys | 2:47 |
| 6 | Freight Train | Pete Seeger | 3:21 |
| 7 | Ticket Agent Blues | Lil’ Son Jackson | 2:33 |
| 8 | John Hurt Medley: My Creole Belle / Payday | Chris Smither | 3:40 |
| 9 | Listen to the Mockingbird | The Kentucky Colonels | 2:21 |
| 10 | Western Plain (When I Was a Cowboy) | Lead Belly | 2:41 |
| 11 | Whoopin' the Blues | Sonny Terry & Brownie McGhee | 2:43 |
| 12 | Well, Well, Well | The Soul Stirrers | 2:49 |
| 13 | John the Revelator | Son House | 1:50 |
| 14 | Little David Play on Your Harp | Merle Travis | 2:19 |
| 15 | Are You Lost in Sin | Jim and Jesse | 2:22 |
| 16 | The Old Crossroads Is Waitin' | Rose Maddox | 3:04 |
| 17 | Bury Me Beneath the Willow | The Carter Family | 2:50 |
| 18 | Lonesome Fiddle Blues | The Nitty Gritty Dirt Band & Vassar Clements | 2:42 |