| # | Title | Length |
|---|
| 1 | "It's a Me, Mario!" | 0:03 |
| 2 | Title Theme | 3:30 |
| 3 | File Select | 0:43 |
| 4 | Peach's Message | 0:07 |
| 5 | Opening | 0:33 |
| 6 | Bowser's Message | 0:04 |
| 7 | Peach's Castle | 1:59 |
| 8 | Toad's Message | 0:04 |
| 9 | Game Start | 0:03 |
| 10 | Main Theme | 2:21 |
| 11 | Stage Boss | 1:20 |
| 12 | Power Star | 0:07 |
| 13 | Course Clear | 0:05 |
| 14 | Star Catch Fanfare | 0:03 |
| 15 | Race Fanfare | 0:04 |
| 16 | Slider | 2:48 |
| 17 | Piranha Plant's Lullaby | 2:17 |
| 18 | Dire, Dire Docks | 3:04 |
| 19 | Correct Solution | 0:04 |
| 20 | Cool, Cool Mountain | 2:57 |
| 21 | Bowser's Road | 2:10 |
| 22 | Bowser's Theme | 2:28 |
| 23 | Bowser Clear | 0:04 |
| 24 | Powerful Mario | 0:53 |
| 25 | Big Boo's Haunt | 3:05 |
| 26 | The Merry-Go-Round | 1:00 |
| 27 | Hazy Maze Cave | 3:44 |
| 28 | Metal Mario | 0:53 |
| 29 | Lethal Lava Land | 2:42 |
| 30 | Game Over | 0:16 |
| 31 | The Endless Stairs | 0:26 |
| 32 | The Final Battle | 2:50 |
| 33 | Final Battle Clear | 0:27 |
| 34 | Ending | 1:15 |
| 35 | Staff Roll | 3:22 |
| 36 | Piranha Plant's Lullaby (piano version) | 2:23 |
