| # | Title | Artist | Length |
|---|
| 1 | Master Assassin | Jesper Kyd | 3:18 |
| 2 | City of Rome | Jesper Kyd | 5:34 |
| 3 | Cesare Borgia | Jesper Kyd | 2:21 |
| 4 | Flags of Rome | Jesper Kyd | 2:36 |
| 5 | The Brotherhood Escapes | Jesper Kyd | 1:59 |
| 6 | Brotherhood of the Assassins | Jesper Kyd | 3:03 |
| 7 | The Pantheon | Jesper Kyd | 3:07 |
| 8 | Villa Under Attack | Jesper Kyd | 2:13 |
| 9 | Echoes of the Roman Ruins | Jesper Kyd | 2:52 |
| 10 | Borgia Tower | Jesper Kyd | 2:14 |
| 11 | Borgia Occupation | Jesper Kyd | 3:04 |
| 12 | Roman Underworld | Jesper Kyd | 3:46 |
| 13 | Countdown | Jesper Kyd | 3:30 |
| 14 | Borgia - The Rulers of Rome | Jesper Kyd | 3:57 |
| 15 | Ezio Confronts Lucrezia | Jesper Kyd | 2:55 |
| 16 | Battle in Spain | Jesper Kyd | 1:38 |
| 17 | Fight of the Assassins | Jesper Kyd | 2:38 |
| 18 | Desmond Miles | Jesper Kyd | 4:41 |
| 19 | VR Room | Jesper Kyd | 2:42 |
| 20 | Apple Chamber | Jesper Kyd | 4:52 |
